Establishment of induction and culture system for hairy roots of Psammosilene tunicoides / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a culture system for Psammosilene tunicoides hairy roots, and provide technological aid for the large-scale production of P. tunicoides material.</p><p><b>METHOD</b>The young leaves and stem segments of sterile plantlets were infected with ACCC10060 strain, and subsequently a culture system suitable for hairy roots growth was further established.</p><p><b>RESULT</b>When explants were co-cultured with ACCC10060 (A600 0.8) on B5 media containing 20 mg x L(-1) Acetosyringo (AS) for 48 h, the hairy roots could be successfully induced, and it could achieve a higher induction rate using young leaves as explants than that of stem segments. The transfected hairy roots possessed the ability of kanamycin resistance and growth on hormone-free media, and synthesis of opines. All above results demonstrated that the present hairy roots originated in the infection of P. tunicoides tissues by ACCC10060 strains. After 35 d culture in liquid hormone-free MS (1/2 strength), the biomass of hairy roots increased 14.11 times (fresh weight) and 8. 39 times (dry weight), respectively, and the content of total saponins in hairy roots reached to 0.857% (DW), by contrast, it's only 0.388% and 0.217% in callus and seedlings respectively.</p><p><b>CONCLUSION</b>Establishment of hairy roots culture of P. tunicoides provided a foundation for industrial production of active components from P. tunicoides culture.</p>

Determination of the Content of Zn in Shenningsan Capsules by ICP-AES Capsules by Atomic Absorption Spectrometry / 中国中医药信息杂志

Objective To establish an ICP-AES method for determination of the content of Zn in Shenningsan Capsules. Methods The sample was digested with HNO3-HClO4, and determined by the ICP-AES method. The determined wavelength was at 213.8 nm, the electric current of the light was set at 10.0 mA, the slit-width was 0.5 nm, the flow rate of acetylerce was 2.0 L/min and the flow rate of air was 9.4 L/min, the hight of burning was 7.5 mm. Results The ion concentration of Zn had the good linear range of 0.2～1.6 ?g/mL, Y=0.054X+0.001 1 (r2=0.999 3). The average recovery of the content of Zn in Shenningsan Capsules was 99.56% with RSD of 2.00%. The content of Zn in Shenningsan Capsules was 68.6 ?g/g. Conclusion The method is simple and reliable. It can be used effectively for the quality control of Shenningsan Capsules.